25-OH-Vitamin D3-BSA Antigen

25-OH-Vitamin D3 is the primary circulating form of Vitamin D3 in the body and a highly bioavailable precursor used as a commercial feed additive in poultry, swine, and cattle farming. It relates directly to animal-derived food safety because its inclusion in animal diets significantly enhances bone strength, immune function, and overall livestock health, which directly increases the concentration of Vitamin D3 and 25-OH-D3 in consumer foods like eggs, meat, and milk. Monitoring its levels is essential to ensure compliance with legal feed supplementation thresholds, validate nutritional fortification claims, and prevent potential hypervitaminosis D (vitamin D toxicity) in animals, which could lead to unsafe, hypercalcemic tissues entering the human food supply.